Use: sufficient for 192 detection reactions (two microplates)
Quality level: 100
Manufacturer/trade name: Roche
Characteristics of greener alternative products
- Design of safer chemicals
- Learn more about the Principles of Green Chemistry.
Greener alternative category: Aligned
Sent in: wet ice
Storage temperature: 2-8°C
We are committed to providing you with greener alternative products that adhere to one or more of the 12 principles of greener chemistry. This product is designed as a safer chemical. The DIG system was established as a sensitive and cost-effective alternative to the use of radioactivity for nucleic acid labelling and detection. There are many publications available proving the versatility of the DIG system, so the use of radiolabeling is no longer the only option for labelling DNA for hybridization.
PCR-ed ELISA, DIG-Detection kit is used for the semi-quantitative detection of digoxigenin (DIG)-labelled polymerase chain reaction (PCR) products. We recommend using the PCR ELISA kit, DIG-Detection in combination with the kit PCR-ed ELISA, DIG-Labeling.
The high specificity of digoxigenin detection allows specific and non-specific amplification products to be distinguished. This is due to the hybridization step with the capture probe. The specificity of this hybridization follows the standard rules for hybridization assays. Strict hybridization in the detection of digoxigenin depends on the length and GC content of the capture probe and the incubation temperature. Temperatures up to 55°C are compatible with the detection of digoxigenin. Under these conditions, with short capture probes (18 to 20 meters), identification of a single base mismatch in the capture oligonucleotide is possible. For this reason, mutations in template DNAs can also be detected by DIG.
The PCR-ELISA DIG detection kit has been used for the detection of Shiga toxin-producing E. coli in food.
1 kit containing 11 components.
This kit is part of a complete PCR ELISA system. By combining it with other reagents and equipment, you can adapt the system for many molecular biology applications. For example:
- If you have a suitable target-specific biotin-labelled capture probe, you can distinguish PCR products that differ by just a single base pair. Therefore, the PCR ELISA system (DIG-labeling + DIG-detection) can be used:
- to detect point mutations, deletions or insertions.
- to classify target sequences (eg, as in HLA typing or cell typing).
- The preparation of suitable DIG-labelled standards and the addition of a colourimetric detection system will allow you to quantify the PCR products.
- The microplate format used by the PCR ELISA system makes the system compatible with automated plate preparation and reading systems.
Labelling: The DIG-labeled PCR products detected with this kit contain alkali-stable digoxigenin-11-dUTP (DIG-dUTP). Said DIG-labeled products can be prepared with the PCR ELISA kit, DIG-Labeling.
Capacity: This size of the PCR ELISA kit, DIG-Detection allows semi-quantitative detection of approx. 50 DIG-labeled PCR products. This number will vary depending on the number of sample dilutions, controls and/or standards used in the assay.
For life science research only. It should not be used in diagnostic procedures.